Download E-books Protocols for Nucleic Acid Analysis by Nonradioactive Probes PDF

By Peter G. Isaac (auth.), Peter G. Isaac (eds.)

This e-book presents a very necessary number of attempted and established protocols for nucleic acid research that keep away from using radioisotopes. Southern (i.e., DNA) and northern (i.e., RNA) blotting protocols are coated intimately, from the way to isolate high quality nucleic acids from plant and animal assets, to using the widest variety of probe platforms (involving digoxigenin, biotin, fluorescein, horseradish peroxidase labeling, and colorimetric and chemiluminescent detection). different strategies handled intimately contain hybridization in situ to chromosomes and transcribed RNA, more moderen and no more traditional applied sciences, akin to Randomly Amplified Polymorphic DNA (RAPD), opposite dot blots, bacterial identity utilizing magnetic bead and dipstick assays, and nucleic acid sequence-based amplification.

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